Apr 13 2017We observed spontaneous Cre activity in both settings Unintended Cre activity is a confounding factor that has a potentially large impact on data interpretation Thus it is important to consider background Cre activity in experimental design 2018 CRE Tech Award Winner Top 75 Workplace Blog 2017 CRE Tech Award Winner Entrepreneur 360 2017 Inc 5000 Most Innovative Tech Company of the Year 2016 Inc 5000 December 10 2018 iOFFICE Awarded in CREtech's 2018 RETAS NEWS ARTICLE November 1 2018 Data is the New Oil: Are You Using Yours? NEWS ARTICLE

Recombinase

Dec 08 2011Specific targeting of dopaminergic and noradrenergic neurons in Th::Cre rats We first generated multiple BAC transgenic rat lines expressing Cre recombinase in tyrosine hydroxylase (TH) neurons (Experimental Procedures) and tested the specificity and potency of these lines for potential optogenetic experiments (Figs 1 – 3) Injection of a Cre-dependent virus in dopaminergic (VTA or

A total of 288 Cre lines have been generated to date 2 Following these efforts many individual laboratories and institutions also produced a variety of Cre drivers (e g Madisen et al 2010 2015 Leo et al 2012) Altogether at least over 630 Cre lines have been deposited at

Despite the upward trend in yield late in the year the 10-year Treasury rate had declined 79 bps between December 31 2018 and December 31 2019 The impact of interest rates on CRE depends on economic growth and spreads between cap and discount rates and interest rates

Allelic replacement in staphylococci is frequently aided by antibiotic resistance markers that replace the gene(s) of interest In multiply modified strains the number of mutated genes usually correlates with the number of selection markers in the strain's chromosome Site-specific recombination systems are capable of eliminating such markers if they are flanked by recombinase recognition sites

CRE cases defined as carbapenem-nonsusceptible Citrobacter freundii Escherichia coli Enterobacter cloacae or Klebsiella pneumoniae infections during January to December 2015 were collected and reviewed from medical records Antimicrobial susceptibility testing and carbapenemase gene identification were performed

Spontaneous recombinase activity of Cre–ERT2 in vivo

Apr 13 2017We observed spontaneous Cre activity in both settings Unintended Cre activity is a confounding factor that has a potentially large impact on data interpretation Thus it is important to consider background Cre activity in experimental design

Cre was inserted under the control of the Adc1 promoter by gap-repair cloning with KpnI-linearized pFA6a-KanMX6-CreERt2-Adc1-2 μ and a PCR product templated by pBS185 ( 12) Finally CreERt2 was removed by ApaI and AscI digestion and religation resulting in the final construct pFA6a2 μ-Adc1Cre

Dec 30 2019The commercial real estate industry (CRE) is advancing in its tech-driven transformation as tenant experience becomes a top priority Deloitte's 2020 CRE Outlook discusses how digital technology is helping CRE executives adapt to tenant preferences and what steps they can take to improve operational performance

The Counselors of Real Estate has identified the current and emerging issues expected to have the most significant impact on real estate for the foreseeable future with the COVID-19 pandemic being the leading concern of the 1 000-member organization COVID-19 Economic Renewal Capital Market Risk Public Private Indebtedness Affordable Housing Flow of People Space Utilization Technology []

Dec 08 2011Specific targeting of dopaminergic and noradrenergic neurons in Th::Cre rats We first generated multiple BAC transgenic rat lines expressing Cre recombinase in tyrosine hydroxylase (TH) neurons (Experimental Procedures) and tested the specificity and potency of these lines for potential optogenetic experiments (Figs 1 – 3) Injection of a Cre-dependent virus in dopaminergic (VTA or

2018 CRE Tech Award Winner Top 75 Workplace Blog 2017 CRE Tech Award Winner Entrepreneur 360 2017 Inc 5000 Most Innovative Tech Company of the Year 2016 Inc 5000 December 10 2018 iOFFICE Awarded in CREtech's 2018 RETAS NEWS ARTICLE November 1 2018 Data is the New Oil: Are You Using Yours? NEWS ARTICLE

CRE are difficult to treat because they do not respond to commonly used antibiotics Occasionally CRE are resistant to all available antibiotics CRE are a threat to public health people icon Patients building icon Healthcare Facilities edit icon CRE Technical Information medical icon Clinicians

CRE cases defined as carbapenem-nonsusceptible Citrobacter freundii Escherichia coli Enterobacter cloacae or Klebsiella pneumoniae infections during January to December 2015 were collected and reviewed from medical records Antimicrobial susceptibility testing and carbapenemase gene identification were performed

Spontaneous recombinase activity of Cre–ERT2 in vivo

Apr 13 2017We observed spontaneous Cre activity in both settings Unintended Cre activity is a confounding factor that has a potentially large impact on data interpretation Thus it is important to consider background Cre activity in experimental design

Allelic replacement in staphylococci is frequently aided by antibiotic resistance markers that replace the gene(s) of interest In multiply modified strains the number of mutated genes usually correlates with the number of selection markers in the strain's chromosome Site-specific recombination systems are capable of eliminating such markers if they are flanked by recombinase recognition sites

Like others in tech its leaders recognized early on how the workplace could more than just a set of bricks and mortar—it could be an active agent in the HR wheelhouse too As more high-profile workplaces like this continue to crop up and inspire others we can expect other organizations also to recognize the power of integrating HR and CRE

Volume 33 March - December 2018 December 2018 issue 4 September 2018 issue 3 June 2018 issue 2 March 2018 issue 1 Financialization and Information Technology – Part II Volume 32 March - December 2017 December 2017 issue 4 Openness and Information Technology September 2017 issue 3 Financialization and Information Technology

Defining and manipulating specific neurons in the brain has garnered enormous interest in recent years because such an approach is now widely recognized as crucial for deepening our understanding of how the brain works When I started exploring the Cre-loxP recombination for brain research in the early 1990s it was written off as a dead-end project by a young fool

Yet over the past 20 years Cre-lox recombination-mediated neurogenetics has emerged as one of the most powerful and versatile technology platforms for cell-specific gene knockouts transgenic overexpression Brainbow imaging neural pathway tracing with retrovirus and CLARITY chemical genetics and optogenetics

A total of 288 Cre lines have been generated to date 2 Following these efforts many individual laboratories and institutions also produced a variety of Cre drivers (e g Madisen et al 2010 2015 Leo et al 2012) Altogether at least over 630 Cre lines have been deposited at

A total of 288 Cre lines have been generated to date 2 Following these efforts many individual laboratories and institutions also produced a variety of Cre drivers (e g Madisen et al 2010 2015 Leo et al 2012) Altogether at least over 630 Cre lines have been deposited at

Volume 44 Number 14 July 22 2020 By Thomas Shircliff CRE and Noah D Shlaes CRE Photo: Josep Suria/Shutterstock Technology Workflow was listed as the #8 issue in the 2020-21 Top Ten Issues Affecting Real Estate by The Counselors of Real Estate In a post-COVID world (and a pre-future pandemic world) the combination of []

Sep 26 2017Article Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals These metrics are regularly updated to reflect usage leading up to the last few days Citations are the number of other articles citing this article calculated by Crossref and updated daily